Thursday , 29 February 2024

Development and Validation of Novel RP-HPLC Method for Simultaneous Estimation of Cobicistat and Atazanavir in Synthetic Mixture

Srinivas Ganta*, Vidyadhara S
Chebrolu Hanumaiah Institute of Pharmaceutical Sciences, Chowdavaram, Guntur – 522018, Andhra Pradesh, India.

A simple accurate and selective Rp-HPLC Assay method was developed for the simultaneous estimation of three drugs Cobicistat (COB) and Atazanavir (ATA) in their tablet formulation. Two drugs were separated on Xterra C18 (5μm, 15cm X 4.6mm) with reverse phase elution of the mobile phase compose of 0.05M Phosphate buffer pH 7 adjusted with orthophosphoric acid: MeOH (30:70%v/v) at a flow rate of 0.8 ml/min. The detection was made at 260 nm. The retention times were 2.53 min for COB and 3.36 min for ATA. The linearity ranges for COB and ATA were 10-50 μg/ml and 20 – 100μg/ml respectively with correlation coefficients 0.999. The proposed method statistically validated with respect to system suitability, linearity, precision, accuracy, specificity, robustness, detection and quantitation limits. The method was found to be accurate, precise, specific and rapid found to be suitable for the quantitative analysis of the drug and dosage form.
Keywords: Cobicistat, Atazanavir, tablet, xterra

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