P. Parveen*, Dr. A. Seetha Devi
Department of pharmaceutics, Hindu College of Pharmacy, Amaravathi Road, Guntur-522002, Andhra Pradesh, India
A B S T R A C T
To develop and characterize bioadhesive gel incorporated Ketorolac tromethamine loaded microspheres in order to sustain, localize and target drug action in periodontal pockets for more than 6hrs. Ketorolac tromethamine loaded microspheres was prepared by emulsion cross linking method using two types of solvents like liquid paraffin or sesame oil by glutaraldehyde cross linking. Tween 80, span 80 were used as emulsifying agents. Gelatin, sodium alginate, HPMC E15 and ethyl cellulose are used as polymers. The prepared microspheres were incorporated in the bioadhesive gel using different polymers like HPMC, NaCMC and Carbopol-934 in various proportions i.e., 300, 400 and 500mg by using triethanolamine as gelling agent. Effect of drug: polymer ratio, stirring rate, glutaraldehyde amount, emulsifying agents ratio and type of polymer on preparation of microspheres was investigated. The microspheres were characterised for % yield (98.5%), % drug loading (85%), particle size (52.14μm), scanning electron microscopy (SEM) and showed 92.8% drug release at the end of 6th hr. The bioadhesive gel was characterised for pH (6.47-6.78), viscosity (55.9-635cps), drug content (92.1-99.7%), bio adhesive strength(2.49-15.7gms) and drug release (98.9%) at the end of 6th hr. Bioadhesive gel loaded with microspheres of Ketorolac tromethamine produces prolonged and localized delivery system for the treatment of periodontitis.
Keywords: Gelatin microspheres, Bioadhesive dosage form, Ketorolac tromethamine, Periodontal pocket, Glutaraldehyde cross linking