Tuesday , 5 March 2024

Bio Activity Guided Separation of Acetyl cholinesterase Inhibitor from Nardostachys jatamansi

K.L. Vegad*1, N.S. Kanaki 1, M.N. Zaveri 1, E.D. Patel2
1Department of Pharmacognosy, K.B. Institute of Pharmaceutical Education and Research Centre, Gandhinagar, Gujarat, India
2Department of Pharmacology, Sharda school of pharmacy, Gujarat, India

Acetyl cholinesterase (AchE) inhibitors are currently still the best available pharmacotherapy for the treatment of various neurological disorders such as Alzheimer’s disease, senile dementia, ataxia and myasthenia gravis, paralytic ileus and glaucoma. Ellman’s method is widely used in-vitro method for evaluation AchE inhibitory activity. In present work methanolic (MeOH) extract of Ficus religiosa (Pipal), Nardostachys jatamansi (Jatamansi) and Tinospora cordifolia (Guduchi) were screened for the AchE Inhibitory activity by Ellman’s method. Donepezil hydrochloride was used as a positive control for AchE inhibitor. Fractionation of MeOH extract of the jatamansi was carried out because of its significant AchE inhibitory activity. Hexane fraction of MeOH extract of jatamansi showed better AchE inhibitory activity (IC50 – 36.41 μg/ml) compared to other fractions. Phytochemical screening of jatamansi showed the presence of alkaloids and steroids which could be responsible for its AchE inhibitory activity.
Keywords: Acetyl cholinesterase inhibitors, Ellman’s method, Donepezil hydrochloride, Nardostachys jatamansi

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