Joysree Das1, Md. Muradur Rahman2,*
1Department of Pharmacy, BGC Trust University Bangladesh, Chittagong, Bangladesh
2Research Scholar, Department of Pharmacy, BGC Trust University Bangladesh, Chittagong, Bangladesh
The present study was designed to investigate antioxidant and thrombolytic properties of chloroform extract of Bacopa monniera (L.), along with phytochemical study for the presence of phytochemical constituents. Phytochemical analyses were found to be positive for carbohydrates and gums, alkaloids and tannins. Antioxidant potential was evaluated using DPPH (2, 2-diphenyl-1-picrylhydrazyl) radical scavenging assays. In DPPH scavenging method, scavenging of DPPH was observed in different concentrations (20, 40, 60, 80, 100, 200, 400, 800μg/ml). Plant extract found to demonstrate significant scavenging activity which was found to increase with concentration of the extract with IC50 value of 16.48µg/ml while IC50 value of the reference ascorbic acid was 1.61µg/ml. Chloroform extract of Bacopa monniera (L.) showed significant (P < 0.001) clot lytic properties in different blood samples. The percent clot lytic activity was compared with water (negative control) and standard enzyme streptokinase (positive control). The mean % of clot lysis for water and streptokinase was found 4.70% and 85.77% respectively. On the other hand the mean percent clot lytic activity of chloroform plant extract of Bacopa monniera (L.) was found 48.39%, which is significant compare with the positive and negative control.
Keywords: Bacopa monniera (L.), DPPH scavenging method, antioxidant potential, streptokinase, clot lytic activity.