Asha Roshan*1, U.K.Jain1, Navneet Kumar Verma2
1Bhopal Institute of Technology & Science-Pharmacy, Bhopal (M.P), India
2Rameshwaram Institute of Technology and management, Lucknow (U.P) India.
Standardization of herbal formulation is essential in order to assess the quality, Purity, Safety & efficacy of the drug based on the amount of their active principles. Pratishyayghnakwath is a polyherbal formulation containing Glycyrrhizaglabra (Roots), Vitisvinifera (Fruits), Cordiadichotoma (Fruits), Viola odorata (Flowers, Leaves), Saccharumofficinarum (Sugar crystals), Pipernigrum (Fruits) as ingredients. It is most widely effective and used in treatment of cough and cold. In present investigation Pratishyayghnakwath was prepared as per official Ayurvedic text “SarSangarh”. The formulation was standardized for organoleptic characters, physico-chemical parameters, phytochemical screening and Thin layer chromatographic studies. A RP-HPLC method was developed for the standardization of Pratishyayghnakwath by qualitative estimation of Glycyrrhetinic acid as markers. The developed method was validated with respect to linearity, precision, accuracy & robustness. The set of parameters were found to be sufficient to evaluate the Pratishyayghnakwath and can be used as reference standards for the quality control/quality assurance purposes.
Keywords: Pratishyayghnakwath (PK), Polyherbal formulation, Standardization etc.