Antidiabetic activity and phytochemical screening of crude extracts of Diospyros malabarica (DM) on Alloxan-induced diabetic Rats

A. Ravi kumar¹*, S. Satyanarayana¹, V. Govardhani¹, Gireesh Jampana¹, Matle Naga Priyanka^2
1Celon Laboratories Private Limited, Aleap Industrial Estate, Near Pragathinagar, Gajularamaram, R.R. Dist. 500090, Telgana, India
²Department of Pharmaceutical Analysis, Narayana Pharmacy College, Nellore, Andhra Pradesh, India

A B S T R A C T
The aim of present study was to evaluate antidiabetic activity of Hydro alcoholic extract of Diospyros malabarica (DM) family:  Ebenaceae,   leaves in Alloxan induced diabetic rats. The Hydro alcoholic extract of Diospyros malabarica (DM) was studied for antidiabetic activity in Alloxan induced diabetic rats by oral administration of extract 200,400mg/kg body weight for 28 days. The effect was compared with oral dose of 150mg/kg Glibenclamide. The determination of blood glucose level by GOD-POD kit method. The result shows the Hydro alcoholic extract of Diospyros malabarica (DM) leaves significantly lowered the blood glucose of hyperglycemic rats. From the toxicity study it was observed that Hydro alcoholic extract of Diospyros malabarica (DM)   was nontoxic up to 2g/kg body weight and phytochemical study showed the presence of phytosterols, flavonoids and glycosides. It is concluded that Hibiscus Cannabinus leaf extract has significant antidiabetic activity, which lowered the fasting blood glucose level in Alloxan induced diabetic rats.
Keywords: Anti-Diabetic activity, Diospyros malabarica, glibenclamide, Wistar rats, hyperglycemic activity, high fat diet, Alloxan