HPTLC, LC-MS Aided Qualitative, Quantitative Phytochemical Screening and Immunosuppressive Activity of Medicinal Plants

Ajam C. Shaikh, Amit Gupta and Sushama R Chaphalkar*
Vidya Pratishthan’s School of Biotechnology (VSBT, Research Centre affiliated to Savitribai Phule Pune University), Baramati, Maharashtra, India

A B S T R A C T
This study analyzed major chemical constituents present in fresh leaves extracts of Strychnus nuxvomica, Emblica officinalis, Ficus racemosa and Syzygium cumini was collected, powdered and then analyzed through HPTLC (high performance thin layer chromatography), LC-MS (liquid chromatography-mass spectrometry) and bioactivity profile such as immunosuppressive activity. Preliminary phytochemical screening of aqueous leaves extract of these medicinal plants showed the presence of chemical constituents like glycosides, flavonoids, terpenoids, phenolic compounds etc. HPTLC finger print analysis of Strychnosnuxvomica, Emblica officinalis, Ficus racemosa and Syzygiumcumini showed the presence of possible number of components and determined its consistent quality of chemical constituents. The experimental conditions as well as general comments on the application of chromatographic fingerprint analysis are discussed. In addition, aqueous extract of these medicinal plants also responsible for immunosuppressive activity against specific protein antigen (HBsAg; 20 µg/ml) i.e. due to decrease in monocytes and granulocytes count.
Keywords: Strychnus nuxvomica, Emblica officinalis, Ficus racemosa, Syzygium cumini, immunosuppressive, LC-MS, HPTLC