Saturday , 2 March 2024

Analytical Method Development and Validation for the Simultaneous Estimation of Acyclovir and Hydrocortisone by RP-HPLC Method in Bulk and Tablet Dosage Form

CH. Shankar*, B. Rajesh
Department of Pharmaceutical Analysis, Vikas College of Pharmaceutical Sciences, Rayanigudem, Suryapet, Nalgonda, Telangana, India-508376

The chromatographic conditions were successfully developed for the separation of Acyclovir and Hydrocortisone by using Agilent C18  Column (250mm x 4.6mm)5µm, flow rate was 1ml/min, mobile phase ratio was  Phosphate buffer PH 2.5:Methanol (65:35 v/v), detection wavelength was 254 nm. The Spectroscopic method was done in solvent using methanol and the instrument lab India 3000+ with UV win software. The instrument used was WATERS HPLC Auto Sampler, Separation module 2690, photo diode array detector, Empower-software version 2. The retention times were found to be 2.113 min and 3.560 min. The % purity of Acyclovir and Hydrocortisone was found to be with in the limits. The linearity study of Acyclovir and Hydrocortisone was found in concentration range of 5µg-25µg and 20µg-100µg and correlation coefficient (r2) was found to be 0.999 and 0.999 respectively, % recovery for Acyclovir and Hydrocortisone was found to be within the limits. %RSD for repeatability and precision was found to be <2. LOD values were 0.001 and 0.005 and LOQ value was 0.004 and 0.015 respectively for Acyclovir and Hydrocortisone.
Keywords: Acyclovir, Hydrocortisone, HPLC.

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