Friday , 25 September 2020

Analytical Method Development and Validation for Sumultaneous Estimation of Asparin and Atrovastatin Calcium in Combind Dosage form by using UV- Spectrophotometry

V. Aparna*, D. Sireesha, Dr. Vasudha bakshi
Department of Pharmaceutical Analysis and Quality assurance, Anurag group of institutions (Formerly Lalitha college of pharmacy), Hyderabad, Telangana, India

A B S T R A C T
A simple, Accurate, precise method was developed for the simultaneous estimation of the Aspirin   and Atorvastatin calcium in Tablet dosage form. Solutions of Aspirin and Atorvostatin Calcium were prepared in different solvents like methanol; ethanol, acetonitrile and UV spectrum of each were recorded by scanning between 200-400 nm. Aspirin was found to be linear in a concentration range of 10-100µg/ml. The absorbances of these solutions were noted at wavelengths 275 and 247 nm, respectively. Calibration curves were plotted using concentration Vs absorbance at wavelength of 275 nm and the slope, intercept and correlation coefficient values were found to be 0.008, 0.007 and 0.999 respectively,  Atorvastatin was found to be linear at a concentration range of 1-10µg/ml. The absorbances of these solutions were noted at 247 and 275 nm, respectively. Calibration curves were plotted using concentration Vs absorbance. At a wavelength of 247 nm, the slope, intercept and correlation coefficient values were found to be 0.001, 0.046 and 0.999, respectively. At wavelength 275 nm, the slope, intercept and correlation coefficient values were found to be 0.006, 0.005 and 0.999, respectively.
Keywords: Aspirin, Atorvastatin , UV-Spectrophotometry

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