Wednesday , 19 June 2024

Analytical Method Development and Validation for Simultaneous Estimation of Asparin and Atrovastatin Calcium in Combind Dosage Form by Using RP-HPLC Method

V. Aparna*, D. Sireesha, Dr. Vasudha bakshi
Department of Pharmaceutical Analysis and Quality assurance, Anurag group of institutions (Formerly Lalitha college of pharmacy), Hyderabad, Telangana, India

A simple, Accurate, precise method was developed for the simultaneous estimation of the Aspirin   and Atorvastatin calcium in Tablet dosage form. Chromatogram was run through Agilent C18, 4.6 mmx150mm, 5mm. Mobile phase containing Methanol and Phosphater buffer in the ratio of 70:30 was pumped through column at a flow rate of 0.8ml/min. Optimized wavelength for Aspirine   and Atorvastatin  was 254nm. Retention time of Aspirine   and Atorvastatin calcium were found to be 2.972min and 3.548 min. %RSD of the Aspirine   and Atorvastatin calcium were and found to be 0.24 and 0.28 respectively. %assay was obtained for Aspirin and Atorvastatin within the limits. LOD, LOQ values are obtained from Aspirin   and Atorvastatin  within the limits respectively.
Keywords: Aspirin, Atorvastatin , RP-HPLC

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