Friday , 21 June 2024

Adenopus Breviflorus Restores Glucose-6-Phosphate Dehydrogenase-Dependent Antioxidant Suppression caused by Clozapine in Female Wistar Rats

Oyabambi Adewumi Oluwafemi1, Okesina Akeem Ayodeji2, Saliu Babatunde Salam1, SalawudeenSadiat Abisola1, Areola Damilare Emmanuel1
1Department of Physiology, Faculty of Basic Medical Sciences,College of Health Sciences, University of Ilorin, PMB 1515, Ilorin, Nigeria.
2Department of Human Anatomy, Faculty of Biomedical Sciences, Kampala International University, Western Campus, P.O BOX 71 Isha-ka/Bushenyi, Uganda.

A  B  S  T  R A C T
Oxidative damage is an important feature of cardiometabolic syndrome. Clozapine (CLO) is a typical antipsychotic medication that alters protein and lipid metabolism with consequent oxidative stress. The G6PD-dependent antioxidant barrier is an important cellular defense against oxidative damage. Nevertheless, Adenopus Breviflorus (AEAB) has been shown to contain beneficial antioxidant phytochemicals and inhibits mitochondrial lipid peroxidation. This study therefore aimed at testing AEAB fruit aqueous extract antioxidant property in CLO-induced redox imbalance. Twenty male Wistar rats weighing between 135-140 g were used for the experiment. They were grouped into Control (CTR; Standard feed and Water), Clozapine only (CLO; 25mg/kg of Clozapine), Extract only (AEAB; 200mg/kg of AEAB) and Clozapine +Extract (CLO+AEAB; 25 mg/kg of Clozapine for first twenty-eight days and AEAB for the last fourteen days), where n=5. All administrations were done orally, and the experiment lasted for forty-two days after a week of acclimatization. The animals were euthanized with 0.2ml of ketamine hydrochloride, blood samples were collected measured for plasma G6PDH, Catalase, Sodium Dismutase, NADPH, GPX, and GSSG using standard spectrophotometric method. The results showed that CLO reduced plasma G6PD, NADPH, GPx, and cardiac NADPH. There was a slight reduction in cardiac G6PD, SOD, GSSG, and GPx with CLO treatment. However, AEAB reversed the alterations caused by CLO treatment in the heart and plasma of animals.AEAB elicits considerable antioxidant activity by limiting the oxidant effects of CLO in the plasma, heart, and plausibly other tissues. AEAB may be considered for therapy in the treatment of oxidative stress-related diseases.
Keywords: Adenopusbreviflorus, Clozapine, Antioxidant, Oxidative damage, Anti-inflammatory

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