C. L. Iwuamadi1, S. O. Iyam2*, C. E. Ibeneme3
1National Assembly Clinic, Area 11, 1 Ikot-Ekpene Close, Garki 1, Abuja.
2Department of Microbiology, Faculty of Biological Sciences, University of Calabar, Calabar, P.M.B. 1115.
3Department of Biochemistry, Faculty of Basic Medical Sciences, University of Calabar, Calabar, P.M.B. 1115.
A B S T R A C T
The antioxidant Gap of serum can be derived using various widely commercially available assays. In this study, three different assays which include total antioxidant status (TAS), uric acid (UA) and albumin were used on the serum samples from five human volunteers to determine the stability of the antioxidants for a short period of 10 days. The samples were stored in various storage conditions which include the room temperature ranging from (28.3oC, 24.6oC, 23.1oC, 24.6oC), fridge temperature (5.3oC, 4.9oC, 4.7oC, 5.7oC) and frozen temperature at -20oC. The various antioxidant assays, TAS, albumin and GAP showed a statistically significant difference with p-values of 0.036, 0.123 and 0.014 respectively, while uric acid with a p-value of 0.610 was not significantly different. In conclusion, the storage of sample for a short period in the above-listed temperature did affect the performance of the total serum antioxidant and the total serum oxidant assay except in uric acid which was not significan.
Keywords: Antioxidant, Gap, stabilities, blood, serum, temperature