D. Dharani Kumari1*, Shaik Obedulla Shariff2, N. Lalitha Kumari3, N. Rami Reddy4
1Department of Chemistry, Krishna Murthy institute of Technology and Engineering, College, Ranga Reddy (Dt), A.P, India.
2Department of physics, Osmania College, Kurnool, AP, India
3Department of physics, Silver Jubliee Government College, Kurnool, AP, India
4Department of Chemistry, S.B.S.Y.M. Degree College, Kurnool, A.P, India
*E-mail: [email protected]
A simple, sensitive, precise and economical visible spectrophotometric method has been developed for the determination of duloxetine hydrochloride in pharmaceutical formulations. The method was based on the formation of chloroform extractable complex of duloxetine hydrochloride with wool fast blue, which shows absorbance maxima at 585 nm against the reagent blank treated similarly. The method obeys Beer’s law in the concentration ranges of 50-250 µg/ml. Validation studies are statistically significant as all the statistical parameters are within the acceptance range (% RSD< 2.0 and S.D. < 2.0) for both accuracy and precision study. High recovery and low % RSD reveals the reliability of the method for quantitative study of the proposed method in tablet formulation.. The proposed method was found to be simple, sensitive, accurate, precise, rapid and economical for the routine quality control application of duloxetine hydrochloride in pharmaceutical formulations.
Key words: Duloxetine hydrochloride, Wool Fast Blue, Spectrophotometer, Formulations