Monday , 24 June 2024

UV-Visible Spectroscopy: Conspectus

Dr. K. Padmalatha, CH. Anupamaswathi*, P. Pavani, P. Sharon, G. Srilakshmi, Ch. Divya, Ch. Lavanya, Yoga Priyanka
Vijaya Institute of Pharmaceutical Sciences for Women, Enikepadu, Vijayawada.

A  B  S  T  R A C T
UV-VIS Spectroscopy is the term used for the analytical estimation of the different types of the solvents and substances. It has been in use for the last 35 years and become the most important analytical instrument in the modern-day laboratory. Spectrophotometer is generally preferred by small-scale industries as the Instrument is inexpensive and the maintenance problems are minimal. The pharmaceutical analysis includes the procedure necessary to seethe “identity, strength, quality and purity” of compounds. It measures a large number of organic and inorganic compounds in a wide range of products and processes – in nucleic acids and proteins, foodstuffs, pharmaceuticals and fertilizers and also includes the raw material analysis and intermediates during the manufacturing process of drugs. The analysis is based on measuring the absorption of a monochromatic light by colorless compounds in the near ultraviolet path of spectrum (200-400nm).

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