Synthesis and Characterization of silver nanoparticles using Phyllanthusacidus leaf extract and its anti-inflammatory activity against human blood cells

P. Anitha¹, P. Sakthivel ^2*
1Assistant Professor, Department of Physics, Roever College of Engineering and Technology, Perambalur, Tamilnadu, India.
²Associate Professor, Department of Physics, Urumu Dhanalakshmi College, Trichy, Tamilnadu, India.

A B S T R A C T
An attempt was made to explore the ethno botanical, economical and biological importance of Phyllanthusacidus. The plant is used for 28 types of remedies like cathartic, emetic, coughs, hypertension, asthma, skin diseases etc and as a food in the raw form. In India, it is found in different states including Tamilnadu and South Indian states as home garden ornaments. Synthesis and characterization of nanoparticles is under exploration due to its wide medical applications and various research interests in nanotechnology. In the current study, the leaf extract of Phyllanthusacidus (Family: Euphorbiaceae) is used for the synthesis of silver nanoparticles. The leaf extract is mixed with AgNO₃ and incubated. The synthesized silver nanoparticles were confirmed by color transformation. It was characterized by UV, FTIR, XRD and TEM. The silver nanoparticles synthesized were generally found in size 1-100 nm. The results showed that the leaf extract is optimum for the synthesis of silver nanoparticles and it is also known to have the ability to inhibit the growth of various pathogenic microorganisms. The average size of synthesized silver nanoparticles is found to be 27.9nm using XRD data by Scherrer’s formula, which is approximately similar as the size obtained in TEM Analysis (28.6nm). In totality, the AgNPs prepared are safe to be discharged in the environment and possibly utilized in processes of pollution remediation. AgNPs may also be efficiently utilized in Anti-inflammatory activity of Pharmaceutical research to obtain better result of plant as shown by our study. The anti-inflammatory activity of silver nanoparticles was tested against human blood cells which confirms that the plant mediated synthesis of silver nanoparticles have a significant anti-inflammatory activity against human blood cells.
Keywords: Phyllanthusacidus, Silver Nanoparticle, UV–Vis Spectroscopy, FTIR, TEM, XRD, Cytotoxity, Human Blood Cells.