Spectrophotometric Determination of Moxifloxacin Hydrochloride using Palladium (Pd-II) ions in micellar medium

Madhuri.D¹*, Chandrasekhar K.B², Devanna N³, Somasekhar G^4
1Department of Pharmaceutical Analysis, Dr. K.V. Subba Reddy Institute of Pharmacy, Kurnool, A. P, India
²Director & Professor, OTPRI-JNTUA, Anantapur, A.P, India-515002
³Professor, Department of Chemistry, JNT University, Anantapur A.P, India-515002
⁴Department of Pharmaceutical Chemistry, Safa College of Pharmacy. Kurnool, A.P, India

A B S T R A C T
A simple ,sensitive and accurate spectrophotometric method was described for the determination of Moxifloxacin hydrochloride (MFX) a broad spectrum flouro quinolone anti bacterial  either  in pure form or in the tablet .The method is based on chelate formation between MFX and Palladium(Pd II) in aqueous media at  pH 8 in presence of surfactant . The complex showed an absorption maximum at 370nm for zero order, Ist derivative at 437 nm and Second derivative at 457 nm respectively with apparent molar absorpitivity of 1.37×10⁴ L-M^-1Cm^-1and sandell’s sensitivity of 0.00364 µg/cm² respectively. The solution of the complex obeyed beer’s law in the concentration range of 1-25 μg/ml for zero order, 10 to50 μg/ml for Ist order and 1to 25 μg/ml for 2^nd order respectively. The limit of detection and limit of quantification were calculated and RSD were calculated. The chelate composition between MFX and Pd(II) ion was found to be 1:1 ratio determined by Job’s continuous method and by Molar ratio method. The proposed method was applied for the determination of MFX in tablets without interference from common excipients. The results obtained by the application of this procedure showed good percentage recoveries.
Keywords: Flouroquinolone, Moxifloxacin, Hydrochloride, Chelate, Aqueous media, Spectrophotometric, Pharmaceutical formulation