V. Motilal Naik, D. Shiva kumar Naik, P. Sathya Sowmya, B. Mohammed Ishaq*, Hindustan Abdul Ahad, Ramesh Dhani
1Department of Pharmaceutical Analysis, Balaji College of Pharmacy, Rudrampeta Bypass, Ananthapuramu-515001, AP, India.
2Dept. of Pharmaceutical Chemistry, Ratnam Institute of Pharmacy, SPSR Nellore, Andhra Pradesh, India-524346
A sensitive, economical, simple precise and accurate UV spectrophotometric method for the determination of metformin hydrochloride in bulk and its tablet dosage form has been developed and validated. The various parameters, such as linearity, precision, accuracy, limit of detection and limit of quantitation were studied according to International Conference on Harmonization guidelines (ICH).The method is accurate, precise (% CV=0.103) and linear within the range 10-40 µg/mL with y = 0.0274x – 0.1369 and coefficient of correlation R² = 0.9579. The limit of detection and limit of quantitation were found to be 0.80 and 2.67 µg/mL respectively. The proposed method was successfully employed for the quantitative determination of metformin tablet dosage form with no interference from any other excipients and diluents.
Keywords: UV spectrophotometric methods, economical metformin hydrochloride