Tuesday , 5 March 2024

Method Development and Validation for Simultaneous Estimation of Ciprofloxacin and Tinidazole in Bulk and Pharmaceutical Dosage Forms

Challa Sudheer1, B. Tirumaleswara Rao2, ­­S. Madhavi2, C. Rambabu*2
1Department of Chemistry, Vikas Degree College (P.G Courses), Vissannapeta, Krishna-521215, A.P, India
2Department of Chemistry, Acharya Nagarjuna University, Nagarjunanagar, Guntur, A.P, India

A simple, rapid, sensitive reverse-phase high-performance liquid chromatography method was developed and validated for simultaneous estimation of ciprofloxacin and tinidazole, at single wavelength of 346nm.chromotographic separation was performed on an enable agilent zorabax (thermo) column (250nmx4.6mm ID particle size 5 um) and a mobile phase consisting of buffer and methanol(70:30v/v) at a flow rate of 1.0 ml/min. The calibration curve was linear(r2≥0.0999) over the concentration range 0.040-0.064 µg/ml.the limit of quantification was 9.737 µg/ml for ciprofloxacin and 9.36 µg/ml for tinidazole no interference was found by the excipients in the synthetic mixture. The proposed methods were validated for international conference on harmonization guidelines for linearity, accuracy, precision, and robustness for estimation of ciprofloxacin and tinidazole in bulk and synthetic mixture, and the results were found to be satisfactory.
Keywords: ciprofloxacin, tinidazole, RP-HPLC Validation.

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