In vitro evaluation of antifungal, seed germination and seedling vigour of aqueous seed extract of Psoralea corylifolia L. against maize seeds

ABOUT AUTHOR
Kiran.B^1*, Lalitha.V² and Raveesha.K.A^3
1Department of Biosciences CMR Institute of Management Studies (Autonomous), Kalyana Nagar, Bangalore -560043, Karnataka State, India
²Department of Studies in Botany and Microbiology, Maharanis Science College for Women, Palace Road, Bangalore-560001
³Department of Studies in Botany, Manasagangotri University of Mysore, Mysore-570 006

Abstract
Aqueous seed extract of P.corylifolia when tested at 10,20,30,40 and 50% concentration for 3, 6,12 and 24 hours of duration against seventeen seed borne of fungi of maize, A. flavus oryzae were completely inhibited at 12 hours of duration in all the concentration tested. A.tamarii were completely inhibited in 6 hours of duration in 10% concentration tested. A. terreus were completely inhibited at 20% concentration in 12 hours of treatment. A.flavus columnaris, Macrophomina phaseolina, Trichoderma viride, Curvularia lunata and Cladosporium cladosporoides  were completely inhibited at 10 concentration in 12 hours of treatment. Phoma lingam, Rhizopus nigricans and Drechslera halodes were inhibited completely at 30% concentration in 3 hours of duration. In seed germination and seedling vigour of maize, maximum germination was observed in 12 hours of treatment at 20% concentration and recorded 88% germination and 1398.5 vigour index. Compared to control, it was recorded 72% germination and 1010.0 vigour index. Significant activity was also observed in 3 and 6 hours of treatment tested at 10,20,30,40 and 50% concentration. In 24 hours of treatment at 40%  and 50% concentration, seed deterioration was observed in all the concentration tested.
Keywords: P.corylifolia, maize, aqueous, fungi, germination, seedling vigour