B. Saidulu*, G. Usha Sree, Dr. V. Umamaheswara Rao
Department of Pharmaceutical Analysis and Quality Assurance, CMR College of Pharmacy, Medchal, Rangareddy, Hyderabad, India
Proteins, peptides, lipids, amino acids, carbohydrates, vitamins and drugs can be separated using chromatography. Chromatography is the separation of a mixture in to individual components sing a stationary phase and mobile phase. Proteins can be separated by using chromatographic techniques like size exclusion chromatography, ion exchange chromatography, affinity chromatography, fast protein liquid chromatography, high performance liquid chromatography, eversed phase chromatography, and electrophoresis. Separation techniques rely on the differences in the solubility, size, charge, and adsorption characteristics of protein molecules. Ion-exchange chromatography is used to separate proteins on the basis of charge. Affinity chromatography utilises ligands, such as enzyme inhibitors, coenzymes, or antibodies, to specifically bind proteins to a solid support. Size of the Protein can be separated by using size exclusion chromatography. Electrophoresis can be used to separate proteins from complex mixtures on the basis of size and charge.
Keywords: Affinity chromatography, Chromatography, electrophoresis, fast protein liquid chromatography high performance liquid chromatography, ion exchange chromatography, reversed phase chromatography, size exclusion chromatography.