Monday , 24 June 2024

Evaluation of In-Vitro Anti-Diabetic Activity of Pulsatilla Nuttalliana (mill.) Leaves extract

Nalamala Kavya*, Y. Prapurna Chandra, C. Madhavi Latha
Department of Pharmacology, Ratnam Institute of Pharmacy, Pidathapolur (V&P), Muthukur (M), SPSR Nellore District – 524 346

A B S T R A C T
The present study is to evaluate the anti-diabetic activity of Pulsatilla nuttalliana leaves extract Diabetes, often referred to by doctors as diabetes mellitus, describes a group of metabolic diseases in which the person has high blood glucose (blood sugar), either because insulin production is inadequate, or because the body’s cells do not respond properly to insulin, or both. In recent years there has been a tremendous increase in demand for herbal drugs due to its safety, efficacy and better therapeutic results Due to its safety, efficacy and better therapeutic results. Due to its economic pricing as compared to synthetic or allopathic drugs which have several therapeutic complication. As we know that everything in this world change time by time since thousands of year the ear was of ayurveda or herbal origin drug. But last few decades it was replaced by allopathic system of medicine, which was rapidly accepted work wide but latter due to its lots of adverse effect and safety profile and the people are more believing in natural origin drug. The leaf extraction has been performed by sequential extraction method using the solvent with increasing polarity order (petroleum ether, ethyl acetate and ethanol) and the active extract was tested by in-vitro antidiabetic screening method. The in-vitro antidiabetic studies have been performed based on the α-amylase inhibition assay. Each extracts were tested for α-amylase inhibition and the extracts with minimum IC50 have been undergone phytochemical screening. The preliminary phytochemical tests was performed to identify the active phytochemicals present in the ethanolic extract of Pulsatilla nuttalliana and showed the presence of Phenols, Flavanoids, Alkaloids, Glycosides, Saponins and Terpenoids. The present study suggested that the isolation of active constituents from ethanolic extract of Pulsatilla nuttalliana leaf and characterize the compounds by using preliminary phytochemical studies.

Keywords: Pulsatilla nuttalliana, in-vitro antidiabetic, Flavanoids, Alkaloids, Glycosides.

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