Madhuri.D1*, Chandrasekhar K.B2, Devanna N3, Somasekhar G4
1Department of Pharmaceutical Analysis, Dr. K.V. Subba Reddy Institute of Pharmacy, Kurnool, A.P, India
2Director & Professor, OTPRI-JNTUA, Anantapur, Andhra Pradesh, India-515002
3Professor, Department of Chemistry, JNT University, Anantapur, Andhra Pradesh, India-515002
4Department of Pharmaceutical Chemistry, Safa College of Pharmacy. Kurnool, Andhra Pradesh, India
A B S T R A C T
A simple ,sensitive and accurate spectrophotometric method was described for the determination of Moxifloxacin hydrochloride (MFX) a broad spectrum flouroquinolone anti bacterial either in pure form or in the tablet .The method is based on chelate formation between MFX and ruthenium(Ru III) in aqueous media at a PH 8 in presence of surfactant . The complex showed an absorption maximum at 367nm for zero order, Ist derivative at 437 nm and Second derivative at 466 nm respectively with apparent molar absorpitivity of 3.66×10 4 L-M-1Cm-1and sandell’s sensitivity of 0.055 µg/cm2 respectively. The solution of the complex obeyed beer’s law in the concentration range of 0.5-5 for zero order,5 to50 for Ist order and 1to 25 for 2nd order respectively. The limit of detection and Limit of quantification were calculated and RSD were calculated. The chelate composition between MFX and Ru(III) ion was found to be 1:1 ratio determined by Job’s continuous method and by Molar ratio method. The proposed method was applied for the determination of MFX in tablets without interference from common excipients .The results obtained by the application of this procedure showed percentage recoveries were 100±0.1463 for zero order, 100.2±0.1065 for Ist order and 100.3±0.1589 for 2nd order respectively.
Keywords: Flouroquinolone, Moxifloxacin Hydrochloride, Chelate, Aqueous media, Spectrophotometric, Pharmaceutical formulation