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Development and validation of UV spectrophotometric method for the estimation of Zidovudine in bulk samples

R. Mounika1, B. Mohammed Ishaq1*, Hindustan Abdul Ahad1, B Faheem1, Ramesh Dhani2 
1Department of Pharmaceutical Analysis, Balaji College of Pharmacy, Anantapur–515001, AP, India.
2Dept. of Pharmaceutical Chemistry, Ratnam Institute of Pharmacy, Nellore, Andhra Pradesh, India-524346
Received: 21 April 2014, Accepted: 28 May 2014, Published Online: 18 July 2014

Abstract
A simple, sensitive, rapid and accurate UV spectroscopic method has been developed for the estimation of zidovudine in bulk samples. The method is based upon determination of Zidovudine at 266 nm in distilled water. Different analytical performance parameters such as linearity, precision, accuracy, LOD and LOQ were determined according to ICH guidelines. The method was found linear between the ranges of 10-120 μg/ml for Zidovudine. The LOD and LOQ was found to be 6.54μg/ml and 21.79μg/ml respectively. Therefore, the proposed method can be used for the routine analysis of zidovudine in bulk samples in quality control laboratories.
Keywords: UV spectroscopic, zidovudine, linearity, precision, ICH guidelines

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