Sunday , 19 May 2024

Development and validation of High Performance Thin Layer Chromatography for estimation of Cefpodoxime Proxetil and Dicloxacillin sodium in combined tablet dosage form

Author Details
1Patel Advaita B*, 2Madhabhai M Patel, 3Bhanubhai N Suhagiya
1Department of pharmaceutical chemistry, Kalol institute of Pharmacy, Kalol, India
2Director and   Principal of Shankersinh Vaghela Bapu Institute of Pharmacy
3Dean at Faculty of Pharmacy, Dharmsinh Desai University, Nadiad

High performance thin layer chromatography for estimation of Cefpodoxime Proxetil and Dicloxacillin Sodium in their combine dosage form was developed and validated. The method was performed on  Camag HPTLC Instrument using Precoated silica gel 60 F254 Aluminum plates (20×10cm) as stationary phase and Benzene: Chloroform : Methanol (5:3:2) as mobile phase at ambient temperature. Detection was carried out at 220 nm. Linearity was observed at concentration range 300-1800ng per band for Cefpodoxime Proxetil and 1500-9000 ng per band for Dicloxacillin Sodium. Correlation coefficient for Cefpodoxime Proxetil and Dicloxacillin Sodium was found 0.996 and 0.999 respectively. Retention factor was found to be 0.24 for Cefpodoxime Proxetil and 0.62 for Dicloxacillin Sodium. The method can successfully applicable to routine analysis.
Keywords: Cefpodoxime Proxetil, Dicloxacillin Sodium, HPTLC

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