Friday , 14 June 2024

Develop a new, simple, fast, rapid, accurate, efficient and reproducible RP-HPLC method and spectroscopic method for the simultaneous analysis of Azelnidipine and Telmisartan

A. Srikanth*, G. Srikanth1, Shaik afroz begum2, Machiraju Sai Kalyan3
*Associate Professor, Dept. of Pharmaceutical Analysis Vasavi institute of pharmaceutical sciences, Kadapa.
1Assistant Professor, Dept. of Pharmaceutics, Vasavi institute of pharmaceutical sciences, Kadapa.
2Assistant Professor, Dept of Pharmaceutical Chemistry, Vasavi institute of pharmaceutical sciences, Kadapa.
3Student, Department of Pharmaceutical Analysis, Vasavi institute of pharmaceutical sciences, Kadapa.

A  b  s  t  r a c t
Introduction: Quality control in manufacturing industries, the monitoring of clinical and environmental samples, the assaying of geological specimens, and the support of fundamental and applied research are the principal applications. Aim: The study aimed to develop a new, simple, fast, rapid, accurate, efficient and reproducible RP-HPLC method and spectroscopic method for the simultaneous analysis of Azelnidipine and Telmisartan. The developed method will be validated according to ICH guidelines. Methods: UV spectrum of 10 µg/ml Telmisartan and 10µg/ml Azelnidipine in diluents (mobile phase composition) was recorded by scanning in the range of 200nm to 400nm. From the UV spectrum wavelength selected as 225 nm. At this wavelength both the drugs show good absorbance. Results and Discussion: The chromatographic conditions were successfully developed for the separation of Azelnidipine and Telmisartan by using Inertsil ODS C18 column (4.6×250mm)5µ, flow rate was 1ml/min, mobile phase ratio was (70:30 v/v) ACN : KH2PO4 ph 3, detection wavelength was 225nm. The instrument used for HPLC , WATERS HPLC Auto Sampler, Separation module 2695, photo diode array detector 996, Empower-software version-2. The retention times were found to be 2.798 mins and 3.587 mins. The % purity of Azelnidipine and Telmisartan was found to be 99.87% and 100.27% respectively. Conclusion: The study concluded that RP-HPLC can be used for routine analysis of Azelnidipine and Telmisartan in API and Pharmaceutical dosage form.

Keywords: UV spectrum, Azelnidipine, Telmisartan, RP-HPLC, spectroscopic method.

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