D. Krishna Priyanka*, C.S. Parameswari, B.V. Ramana, G. Nagarajan
Dr. K.V. Subba Reddy Institute of Pharmacy, Kurnool, Andhra Pradesh, India-518218
A B S T R A C T
The aim of this work is to design and fabricate a collagen based dermal scaffold containing Terminalia Arjuna extract for the improvement in process of wound healing. For this purpose Collagen was isolated from bovine Achilles tendon and tested for its confirmation of presence, purity and sterility. The physicochemical compatibility between collagen and Terminalia Arjuna extract was studied by FT-IR and results obtained suggested no interaction and they were compatible. Collagen based Scaffolds were formulated using different concentrations (1%w/v, 1.5%w/v & 2%w/v) of Terminalia Arjuna extract (TAEICDS- Terminalia Arjuna extract incorporated collagen dermal scaffolds). The prepared scaffolds were subjected to physical and biochemical examinations. Microshrinkage Temperature of the scaffolds containing 1%w/v & 1.5%w/v & 2%w/v of TAEICDS were found to be 680C,690C, 710C & 720C respectively indicating their hydrothermal stability. Wound healing studies on Male Wister Rats were performed for a period of 7 days and it was observed that the 2 %w/v TAEICDS treated rats possessed higher amount of hydroxyl proline content. Further better wound healing activity was observed in the 2%w/v TAEICDS treated rats (78%) when compared to others.
Keywords: Collagen, Terminalia Arjuna extract, Anti oxidant Efficiency, Wound Healing.