Monday , 6 February 2023

Dengue virus serotype 2 proliferation using different cell substrates

Gangadharam Poli1, Suresh Kumar Chitta1, Madhusudana Pulaganti3, Prasad Reddy S Hari4, CM Anuradha1,2*
1Department of Bio-Chemistry, Sri Krishnadevaraya University, Anantapuramu, Andhrapradesh, India- 515003.
2Dept of Biotechnology, Sri Krishnadevaraya University, Anantapuramu, Andhrapradesh, India- 515003.
3Multidisciplinary Research Unit, SV Medical College, Tirupati, Andhrapradesh, India-517507
4Dept of Biotechnology, Koneru Lakshmaiah, Education Foundation, Guntur, Andhrapradesh, India- 522502

A B S T R A C T
Dengue viruses are infectious agents that can replicates only within a living host cells. The advanced cell culture techniques are more appropriate methods to cultivate any viruses in laboratory conditions. By using these cell culture methods, viruses can be produced certainly in high titres. In biotechnological applications, adherent and monolayer cell cultures are using extensively to grow the viruses. Based on the literature, the viruses are non-susceptible to some kind of cell substrates, which results in poor viral growth.  Dengue virus serotype-2 (DENV-2) susceptibility is studied with Vero, CHO and HeLa cell lines. These cell lines are widely used in different viral products manufacturing and analytical methods for viral quantification assays. Based on the observed cytopathic effect on monolayer confluency and obtained viral harvest titers, it is concluded that Vero cell substrate is more susceptible to dengue virus (DENV-2) than the other two cell lines.

Keywords: Infectious disease, Virology, Cell cultures, Dengue virus, Cell susceptibility, Cell permissively

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