Monday , 15 August 2022

Biodegradation of Indigo Blue Dye using the Soil and Sludge Isolate of the Effluent Run off Site

C. Initha Lebanon Ebency1*, S. Rajan2, A.G. Murugesan3
1Department of Microbiology, Sree Narayana Guru College, K.G. Chavadi, Coimbatore–641105
2Department of Microbiology, M.R. College of Arts and Science, Manargudi, Tamilnadu, India
3Sri Paramakalyani Centre for Environmental Sciences, Manonmanium Sundaranar University, Tirunelveli, T.N, India

ABSTRACT
Cloths are of high demand from centuries as it implies the personality of a human being; hence the textile industry has established a wide empire over the time. Textile industries consume large volumes of water and chemicals during manufacture and processing. Colour is the first contaminant to be recognized in textile waste water and has to be removed before discharging into water bodies or onto land.  Azo dyes account for 60–80 % of the dyes consumed in the textile processing. Indigo blue dye is used mostly in all jean manufacturing textile industries. To decolourise the indigo blue dye, the soil and sludge was collected from different jean manufacturing industries in Coimbatore district. Indigo blue dye degrading organisms were screened using Zhou and Zimmerman (ZZ) screening media after serially diluting the collected samples. After screening and isolating the best dye decolouriser from the samples, optimizations of the dye degrading conditions were carried out. Different factors like temperature, pH, carbon source, nitrogen source, metallic salts, inoculam size and time course of decolourization was selected as optimization parameters. The best degrader was found to be Bacillus sp. From the optimization analysis, the suitable optimized condition for the isolate, Bacillus sp. was identified as sucrose (1%), ammonium chloride (0.25%), temperature (45 C), pH (8.0), potassium dihydrogen orthophosphate (84.96%) and inoculum dose (2%). The time required for effective decolourization (98%) of the indigo blue dye was estimated to be 48 hours. Thereby the results suggest Bacillus sp. isolated from the effluent discharge showed a potential of degrading the selected indigo dye at a faster rate and these properties are found to be useful for the bioremediation of textile industry effluent.
Keywords: Indigo blue dye, Bacillus sp. , Zhou and Zimmerman (ZZ) screening media, pH, carbon source, nitrogen source, metallic salts, inoculam size and time course of decolourization.

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