1Kranthi Ponugoti*, 1Rohitha Kesireddy, 2Kartheek.S, 3K.Satish
1Malla Reddy College of Pharmacy, Hyderabad, India
2Annamalai University, Tamilnadu, India
3Vikascollege of Pharmaceutical Sciences, Suryapet, India
Antitumor activity of wheatgrass (400 mg/kg) was evaluated against the MCF-7 and Caco2 cell lines and these cell lines induced breast and colon cancer in mice. Materials and Methods in used Invitro cytotoxicity of wheatgrass was observed by MTT assay. After 24 hr of tumor inoculation, the wheatgrass was administered daily for 30 days. After administration of the last dose followed by 18 hr fasting, mice were sacrificed for observation of antitumor activity. The effect of wheatgrass on cell proliferation (invitro) was estimated. The change in body weight, abdominal circumference of tumor bearing hosts and simultaneous alterations in the hematological profile, serum (SGPT, LDH, ALP and glucose) and liver biochemical parameters (lipid peroxidation, GSH and antioxidant enzymes) were estimated. The changes in Carcinoembryonic antigen and ferritin levels were estimated. The wheatgrass inhibited the cell growth (invivo) and exhibited apoptosis of cell lines. The wheatgrass maintained the abdominal circumference and body weight of tumor bearing mice. Hematological profile reverted towards normal levels in wheatgrass treated mice. Treatment with wheatgrass restored the serum biochemical parameters towards normal levels and decreased the levels of lipid peroxidation and increased the levels of reduced glutathione and other antioxidant enzymes (CAT and GPx). The wheatgrass treatment restored the Carcino embryonic antigen and ferritin levels in tumor induced mice. The wheatgrass exhibited antitumor effect by modulating the hematological parameters, lipid peroxidation and augmenting antioxidant defense system in tumor bearing mice.
Keywords: Cancer Tumor, Invitro & Invivo Studies, Cell line, Plant Extracts, Animals (Mice).