Wednesday , 24 July 2024

Analytical Method Development and Validation for the Simultaneous Estimation of Telmisartan and Hydrochlorothiazide by UV-Spectrophotometric Method in Bulk and Tablet Dosage Form

L. Kanaka Lakshmi*, S. Harshini, Dr. Vasudha Bakshi, D. Sireesha, Akiful Haque
Deparment of Pharmaceutical Analysis, School of Pharmacy, Anurag Group of Institutions, Ghatkesar, Ranga Reddy, Telangana-501301, India. 

The Spectroscopic method was successfully developed for the separation of Hydrochlorothiazide and Telmisartan by using as solvent methanol, detection wavelength were at 271nm and 296 nm. The Spectroscopic method was done in solvent using methanol and the instrument lab india 3000+ with uv win software. The linearity study of Hydrochlorothiazide and Telmisartan was found in concentration range of 2µg/ml-12µg/ml and 5µg/ml-30µg/ml and correlation coefficient (r2) was found to be 0.999 and 0.997, % recovery was found to be 98.56% and 99.96%, %RSD for repeatability was 1.2, % RSD for intermediate precision was 1.9. The precision study was precision, robustness and repeatabilty. LOD value was 3.72 and 0.0242 and LOQ value was 7.40 and 0.0202 respectively. The Proposed method is validated and it is useful for the determination and estimation of hydrochlorothiazide and Telmisartan in its bulk and pharmaceutical dosage form.
Keywords: Telmisartan, UV visible spectrophotometer, Hydrochlorothiazide, LOD, LOQ.

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