Thursday , 22 February 2024

Analytical Method Development and Validation for the Simultaneous Estimation of Telmisartan and Carvedilol by RP-HPLC Method

Maka Saroja Rani1*, Gope Edward Raju2, Kandregula Uma Maheswari3, Doonaboyina Raghava4, Kavala Nageswara Rao5
Department of Pharmaceutical Analysis, K.G.R.L College of Pharmacy, Bhimavaram-534201, Andhra Pradesh, India

A b s t r a c t
A simple precise and accurate reverse phase high performance liquid chromatographic technique was developed and validated for the simultaneous estimation of Telmisartan and Carvedilol in a combined dosage form Symmetry Agilent C18 (4.6*150mm) 5µm column in isocratic mode was used with the mobile phase comprising of Water and Methanol in the ratio of 40:60v/v, the flow rate was set at 1ml/min. The analyte was monitored with dual wavelength UV detector at 255nm. The retention time of Telmisartan and Carvedilol was found to be 2.551 and 4.879 min respectively. The linearity range was found to lie from 10µg/ml to 50µg/ml of Telmisartan, 20µg/ml to 100µg/ml of Carvedilol. Percentage recoveries were obtained in the range of for Telmisartan 98.8% and for Carvedilol 98.5%. The proposed method is precise, accurate, selective, reproducible and rapid for the simultaneous estimation of Telmisartan and Carvedilol in combined form.
Keywords: Telmisartan, Carvedilol, UV, HPLC

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