Sameera Banu1, D. Naresh2, Gampa Vijay Kumar3*
1KGR Institute of Technology and Management, Rampally, Kesara, Rangareddy, Telangana, India.
2KGR Institute of Technology and Management, Rampally, Kesara, Rangareddy, Telangana, India.
3Professor and Head, Dept.of Pharmacy, KGR InstituteofTechnology and Management, Rampally, Kesara, Rangareddy, Telangana, India.
High performance liquid chromatography is at present one of the most sophisticated tool of the analysis. The estimation of Carbidopa and Olanzapine was done by RP-HPLC. The Phosphate buffer was pH 4.5 and the mobile phase was optimized with consists of Phosphate buffer: Methanol PH 4.5 (20:80 v/v). Kromosil C18 Column (250mm x 4.6mm)5µg or equivalent chemically bonded to porous silica particles was used as stationary phase. The detection was carried out using UV detector at 254nm. The solutions were chromatographed at a constant flow rate of 1ml min-1. The linearity range of Carbidopa and Olanzapine were found to be from 100-500 mg/ml of Carbidopa and 1-5mg/ml of Olanzapine . Linear regression coefficient was not more than 0.999. The values of % RSD are less than 2% indicating accuracy and precision of the method. The percentage recovery varies from 98-102% of Carbidopa and Olanzapine. LOD and LOQ were found to be within limit.The results obtained on the validation parameters met ICH and USP requirements .it inferred the method found to be simple, accurate, precise and linear. The method was found to be having suitable application in routine laboratory analysis with high degree of accuracy and precision.
Keywords: Kromosil C18, Carbidopa and Olanzapine, RP-HPLC