Friday , 24 May 2024

Analytical Method Devalopment and Validation for the Simultaneous Estimation of Ephidrine and Theophylline by RP- HPLC Method

N. Karnakar1, H. Ramana2, Parameshwar3, R. Narsimha Rao4, R. Vasanthi5
1,,3,4Venkateshwara Institute of Pharmaceutical Sciences, Charlapally, Nalgonda, Telangana.
2Department of Pharmacy, College of Health Sciences and Medicine, Wolaita Sodo University, Ethiopia.
5School of Pharmacy, GITAM Deemed to be University, Hyderabad

Another technique was set up for concurrent assessment of Theophylline and Etofylline by RP- HPLC strategy. The chromatographic conditions were effectively created for the partition of Theophylline and Etofylline by utilizing Xterra C18 5µm (4.6*250mm) section, stream rate was 1ml/min, portable stage proportion was Phosphate support (0.05M) pH 4.6: ACN (55:45%v/v) (pH was changed with orthophosphoric corrosive), location frequency was 255nm. The % virtue of Theophylline and Etofylline was discovered to be 100.7% and 101.4% individually. The framework reasonableness boundaries for Theophylline and Etofylline, for example, hypothetical plates and following element were discovered to be 1.3, 5117.5 and 1.4, 3877.3 the goal was discovered to be 8.0. The diagnostic strategy was approved by ICH rules (ICH, Q2 (R1)). The linearity concentrate for Theophylline and Etofylline was found in focus scope of 1μg-5μg and 100μg-500μg and relationship coefficient (r2) was discovered to be 0.999 and 0.999, % mean recuperation was discovered to be 100% and 100.5%, %RSD for repeatability was 0.2 and 0.4, % RSD for middle accuracy was 0.5 and 0.1 individually. The exactness study was exact, vigorous, and repeatable. LOD esteem was 2.95 and 3.04, and LOQ esteem was 9.87 and 10 separately.
Keywords: Xterra C18, Theophylline, Etofylline, RP-HPLC

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