Khan Majid Shabbir1,I. Sarath Chandiran*2, P.D. Chaudhari3
1Research Scholar, Prist Univesity, Vallam, Thanjavur, Tamilnadu, India
2Professor, Ratnam Institute of Pharmacy, Pidathapolur, SPSR Nellore-524346, Andhra Pradesh, India
3Principal, Modern College of Pharmacy, Nigdi, Pune-44, Maharashtra, India.
A B S T R A C T
The present study was aimed to evaluate phytochemical constituents and the safety of methanolic leaf extract of Bombax ceiba (MEBC) by determining their potential toxicity after acute and 28-day repeated dose administration in Wistar Albino rats. The phytochemical analysis was done by standard laboratory grade reagents. Acute and 28-day repeated dose oral toxicity studies were performed by the following Organisation for Eonomic Co-operation and Development (OECD) test guidelines 423 and 407 respectively. The phytochemical screening revealed the presence of steroid, alkaloid, tannin, phenols, flavonoids, in methanol soluble fractions. Fats and fixed oils were undetected in the extracts. In acute toxicity study no treatment related death or toxic signs were observed with MEBC administration. In repeated dose study no significant differences in body weight changes and hematology was observed between control and MEBC groups. Conversely there was a decrease in serum glucose and cholesterol levels and an increase in protein levels in treated rats compared to control. No gross pathological findings and difference in relative organ weights were observed between control and treated rats. Histopathological examination revealed no abnormalities with the test drug treatment. In conclusion Bombax ceiba was found to be non toxic in tested doses and experimental conditions.
Keywords: Bombax ceiba, OECD, MEBC