Acute and Subacute toxicity study of Guanfacine hydrochloride in Rats by Oral route

U. Venkatesh¹*, Sri Rama Radha¹, P. Suresh¹, G. Sravan Reddy^2
1St John’s College of Pharmacy, Yemmiganur, Kurnool, Andhra Pradesh
²Hi-Q Herbals, Kothapet, Hyderabad, Telangana

A B S T R A C T
Guanfacine is a sympatholytic. It is a selective α_2A receptor agonist. These receptors are concentrated heavily in the prefrontal cortex and locus ceruleus. Guanfacine lowers both systolic and diastolic blood pressure by activating the central nervous system α_2A   norepinephrine autoreceptors, which results in reduced peripheral sympathetic outflow and thus a reduction in peripheral sympathetic tone. The present investigation in wistar rats to evaluate the pre-clinical safety of Guanfacine Hydrochloride USP manufactured. The acute study design involves single group consisting of 10 animals (5 males & 5 females). Whereas sub-acute study design involves four groups consisting 12 animals per group (6 males & 6 females). The vehicle control group received sterile water for injection, whereas low dose, middle dose and high dose group animals have received test item at various dose levels. The test item has been administered orally in a constant volume 1 ml/kg for 28 days. The results of single dose 14 days toxicity study indicated no lethality in all animals and repeated dose 28 days toxicity study indicated no lethality in control and treated group animals. There were no significant changes recorded in haematological, biochemical and histopathological parameters under experimental conditions in wistar rats.
Keywords: Cytosine, Biosensor, Amperometry, Polymer, Glassy carbon electrode