Friday , 19 July 2024

A Simple UV Spectroscopic Method for the Determination of Ritonavir in Bulk and Tablets

B. Mohammed Ishaq1, S. Muneer1*, H. Ruksana1, Dr. Hindustan Abdul Ahad1Dr. K. Vanitha Prakash2 
1Department of Pharmaceutical Analysis, Balaji College of Pharmacy, Anantapur-515001, A. P, India
2Department of Pharmaceutical Analysis, SSJ College of Pharmacy, Hyderabad, A.P. India
Received: 10 April 2014, Accepted: 18 May 2014, Published Online: 18 July 2014

A simple, economic, accurate UV method was developed for the estimation of Ritonavir (RV) in bulk and tablet dosage form. Water and methanol in the ratio of 50:50 v/v was used as a diluent to dissolve RV. The drug mixture was sonicated for 2 mins for the enhanced solubility. The absorptions were observed at 240.0 nm, which was selected for the further analysis of RT in bulk and its tablet dosage forms. The proposed method was validated according to ICH guidelines. The method showed high sensitivity with linearity range from 10 to 80 μg/ml (r2=0.999) at 240.0 nm. The limit of detection (LOD) was found to be 50 μg/ml and the limit of quantization (LOQ) was determined as the lowest concentration was found to be 200 μg/ml. The reports expressed that the proposed method was found to be simple, precise, accurate and rapid for the estimation of RT in bulk and tablet dosage form using UV spectroscopy.
Keywords: Ritonavir, methanol, water, UV spectroscopy, ICH guidelines

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