Dr. Hareesh Dara*, D Siva Dinesh, Dr. Thatipelli Ravichander
St John College of Pharmcy, Hasanparthy, Hanamkonda, Telangana, India
A B S T R A C T
The lemon [Citrus limon (L.) Rutaceae] exhibits many important natural chemical components, including citric acid, ascorbic acid, minerals and phenolic compounds, such flavonoids. Although their biological properties have always been associated with their content of vitamin C, it has recently been shown that flavonoids and other nutrients and non-nutrients (vitamins, minerals, dietary fiber, essential oils and carotenoids). Therefore, their health-promoting effects, such as obesity, diabetes, blood lipid lowering, cardiovascular diseases, brain disorders and certain types of cancer, have been associated with their contents, especially vitamin C and flavonoids, due to their natural antioxidant characteristics. Therefore, this work was designed based on phytochemical constitution such as flavonoids in Citrus limon peel extracts because flavonoids are rich in the treatment for evaluation of analgesic and anti-inflammatory activity in rats. Fresh and crushed peel of Citrus limon were collected and then extracted with different solvents such as water, alcohol and methanol at the doses of 100 mg/kg, 200 mg/kg body weight in experimental animal models. Analgesic activity was evaluated by Hot-plate and tail –flick method in albino wistar rats; acute and chronic anti-inflammatory activity was evaluated by carrageenan-induced paw oedema and formalin-induced paw edema in Wistar albino rats. Diclofenac sodium and indomethacin were employed as reference drugs for analgesic and anti-inflammatory studies, respectively. In the present study, the aqueous, alcoholic and methanolic extracts of Citrus limon demonstrated significant analgesic and anti-inflammatory activities in the tested models.
Keywords: citrus limon, hot-plate, tail-flick.