K. Krishna Chaitanya*1, K. Kamalakara Rao1, Y.N. Sastry1, Dr. S.B. Padal2, Dr. A. Rajya lakshmi3, Dr. D. Govinda Rao1
1Department of Biochemistry, GITAM Institute of Science, GITAM University, Visakhapatnam, Andhra Pradesh, India
2Associate Professor, Department of Botany, Andhra University, Visakhapatnam, Andhra Pradesh, India
3Director, Bio world research technologies, Hyderabad, Telangana, India.
A B S T R A C T
In the present study investigation was carried out to assess the in vitro anti-inflammatory, antioxidant activities and phytochemical analysis of Mesua ferrea bark ethyl acetate extract. Novel anti-inflammatory and antioxidants screened from medicinal plants used as a medication for treatment of diseases without having any side effects. The anti-inflammatory activities of Mesua ferrea bark extracts was evaluated by in vitro 5-LOX and HRBC membrane stabilization assay. Among all the extracts MFBEE exhibited significant 5-LOX inhibitory activity and shown protective effect on the HRBC membrane stabilisation. ROS and RNS mediates oxidative stress has been implicated in pathogenesis of several chronic diseases. MFBEE exhibited significant DPPH, ABTS, NBT and NO free radical scavenging activity. The phytochemical analysis of Mesua ferrea bark ethyl acetate extract revealed the presence of high amount of phenolic content, flavonoids, terpenoids and coumarins which may be responsible for anti-inflammatory and antioxidant activities of Mesua ferrea bark ethyl acetate extract.
Keywords: Mesua ferrea, anti-inflammation, antioxidant, membrane stabilization, 5-Lipoxygenase, phytochemicals, reactive oxygen species, reactive nitrogen species, oxidative stress, Total phenolic content.