Sunday , 22 May 2022

Validation of UV-HPLC method for simultaneous quantification of organic acids in disinfectants for haemodialysis machines

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1Mahmood Ahmed*, 1Muhammad Abdul Qadir, 1Shabnam Shahzad, 1Rabia Waseem, 2Muhammad Saqlain Tahir
1Institute of Chemistry, University of Punjab, Lahore-Pakistan
2Highnoon Laboratories Limited, Lahore- Pakistan

A UV-HPLC method was validated for quantification of citric, lactic and malic acidsin disinfectants. The chromatographic separation was performed at 40°C by using mobile phase comprised a mixture (90:10) of potassium phosphate monobasic (50mM) and acetonitrile and pH was adjusted to 2.8 with ortho phosphoric acid, on C18-5µm and 1000A pore size reverse phase column (250mm×4.6mm) at λ=210 nm using UV-Vis detector with 1.25ml/min flow rate. ICH guidelines were adopted to perform the validation studies that the analytical method’s characteristics are reliable throughout the time during analysis. Validation parameters showed efficiency and adequate linearity with correlation coefficient (r2) 0.9997, 0.9998 and 0.9997for 20-80 μg/ml citric acid, 2-8μg/ml lactic acid and malic acid respectively. The repeatability and intermediate precision (%RSD<2) indicated the method reproducibility. The limits of detection (LOD) 1.93μg/ml, 0.11μg/ml,0.21μg/ml and the limit of quantification (LOQ) were 6.46μg/ml, 0.34μg/ml, and 0.68μg/ml for citric acid, lactic acid and malic acid respectively.% Recovery ± SD for citric, lactic and malic acids was98.22±0.24 – 100.3±0.03, 98.26±0.03 – 101.26±0.03 and 97.42±0.03 – 100.5±0.07 respectively for three spikes levels.
Key words: Organic acids, HPLC, Disinfectants, Haemodialysis 

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