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Tag Archives: RP-HPLC

Development and Validation of Simultaneous Determination of Vitamins B1, B2, B3, B6 and Folic Acid in Multivitamin Tablet by RP-HPLC

A. Sankara Avudaiappan1, A. Chenthilnathan1* and S. Vikraman2  1Department of Pharmaceutical Chemistry, Manonmaniam Sundaranar University, Tirunelveli – 627 012, Tamil Nadu, India. 2Apex Laboratories Private Limited, Alathur, Kancheepuram District-603110 Tamil Nadu, India. Received: 29 April 2014, Accepted: 15 June 2014, Published Online: 27 July 2014 Abstract A simple, efficient and reproducible RP-HPLC method for simultaneous determination of vitamins B1 (Thiamine mononitrate), ... Read More »

Validated RP-HPLC Method for Simultaneous Estimation of Lisinopril and Hydrochlorothiazide in Combined Dosage forms

Author Details P. Nagaraju*, G. Indira Priyadarshini, SA. Bilal Ahmed and V. Siddhartha Department of Pharmaceutical Analysis, Hindu College of Pharmacy, Amaravathi Road, Guntur–522 002, Andhra Pradesh, India. Abstract A rapid and sensitive reverse phase high performance liquid chromatography (RP-HPLC) method has been developed for simultaneous estimation of Lisinopril and Hydrochlorothiazide. Chromatographic separation was achieved on reverse phase Agilent LC 1100 ... Read More »

Method development and validation of telmisartan and amlodipine besylate by RP-HPLC in Tablet Dosage form

Author Details MD. Ashraf Basha*, K. Mangamma, G. Mani kumar  Dept of Pharmaceutical Analysis and Quality Assurance, JNTU School of Pharmaceutical Sciences, Kakinada Abstract A simple, precise, accurate and rapid reverse phase high performance liquid chromatographic method had been developed for simultaneous estimation of Telmisartan and Amlodipine Besylate in tablet dosage form. SymmetryC18 4.6 x 250mm, 5m particle size was ... Read More »

Validated RP-HPLC Method for the Quantification of Anidulafungin In Bulk Sample and Parenteral Dosage Form and its Application to forced Degradation Studies in Bulk Sample

About author Lohita M*, Jaya Preethi P, Swetha K, Vengal Rao P, Naresh D, Amrutha V Department of Pharmacy, Sree Vidyanikethan College of Pharmacy, Sri Sainath nagar, Tirupati-517102. E-mail:  lohitapharma11@gmail.com Abstract A simple, selective, precise and stability-indicating high-performance liquid chromatographic method of analysis of Anidulafungin in pharmaceutical dosage form was developed and validated. The solvent system consisted of acetonitrile: water: 0.1% v/v ... Read More »

Method development and validation by RP-HPLC for the estimation of Zolmitriptan in bulk and its dosage form

About author D. Samson Israel*, D.Gowri Sankar,  K.Krishna Chaitanya, K.Balaram Kumar, K.Vinod University college of Pharmaceutical sciences, Andhra University, Visakhapatnam, A.P E-mail: samson.pharma@gmail.com Abstract Zolmitriptan is used in the acute treatment of migraine attacks. It was quantified by reverse phase HPLC equipped with Sunniest (C18, 150 x 4.6 mm, 5.0 µ) column and mobile phase using pH 8.5 buffer and methanol as organic modifiers ... Read More »

Method development and validation for the simultaneous estimation of Nebivolol hydrochloride and S (-) Amlodipine besilate in tablet dosage form by RP – HPLC

About author Prabhakaran. V.*, Sekar. S., Baskar.E., Raghuprasath. V.R. Formulation Research and Development Department, The Madras Pharmaceuticals, Karapakkam, Chennai, Tamil Nadu, India. Email: rnd@madraspharma.com ABSTRACT High Performance Liquid Chromatography method was developed and validated in a simple, specific and precise manner for Nebivolol HCl and Amlodipine besilate in a combined solid dosage form. Nebivolol hydrochloride, a long acting, cardioselective β ... Read More »

METHOD DEVELOPMENT AND ITS VALIDATION FOR QUANTITATIVE SIMULTANEOUS DETERMINATION OF DEXAMETHASONE AND GATIFLOXACIN IN OPHTHALMIC SOLUTION BY RP-HPLC

About author : Ankit Agarwal*, Subhash Dadhich, Sunil Kumar Tiwari, Kashyap Nagariya R & D Division, Ahlcon Parenterals (I) Ltd., Bhiwadi, Rajasthan, India *e-mail: ankitagarwal005@gmail.com ABSTRACT : To develop a simple, rapid and accurate HPLC method for simultaneous quantitative determination of Dexamethasone and Gatifloxacin in ophthalmic solution. Chromatographic separation was achieved with PDA detector using Inertsil C18, 250 x 4.6mm, ... Read More »

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