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Tag Archives: HPLC

Analytical Method Development and Validation for the Simultaneous Estimation of Metformin and Gemigliptin by RP-HPLC Method in Bulk and Pharmaceutical Dosage Form

Y. Krishna Reddy*, Kedhareshwari, Y. Harikha, V. Harikrishna, Muzahid, K. Gopi Bomma Institute of Pharmacy, Khammam, Telangana, India A B S T R A C T The chromatographic conditions were successfully developed for the separation of Metformin and Gemigliptin by using RP-HPLC. The Phosphate buffer was pH 3.0 and the mobile phase was optimized with consists of Methanol: Phosphate buffer ... Read More »

Analytical Method Development and Validation for the Simultaneous Estimation of Ezetamibe acid and Simvastatin by RP-HPLC Method in Bulk and Pharmaceutical Dosage Form

Y. Krishna Reddy*, B. Ratna Kumari, M. Sampath, B. Naresh, CH. Kavya, CH. Ramadevi Bomma Institute of Pharmacy, Khammam, Telangana, India  A B S T R A C T The chromatographic conditions were successfully developed for the separation of Ezitamibe and Simvastatin by using Agilent C18 5µm (4.6*250mm) column, the flow rate was 1ml/min, mobile phase ratio was Methanol: ACN  ... Read More »

Analytical Method Development and Validation for the Estimation of Ceritinib by RP-HPLC Method in Bulk and Pharmaceutical Dosage Form

Krishna Reddy*, K. Pranaya, K. Babu, MA. Tanjeema, N. Ravi kumar, Udayasri Bomma Institute of Pharmacy, Khammam, Telangana, India A B S T R A C T The chromatographic conditions were successfully developed for the separation of Ceritinib by using Kromosil C18 4.5×150 mm 5.0 µm, flow rate was 0.8ml/min, and mobile phase ratio was 65:35% v/v methanol: water, detection ... Read More »

Analytical Method Development and Validation for the Estimation of Pantoprazole by RP-HPLC Method in Bulk and Pharmaceutical Dosage Form

Y. Krishna Reddy*, D. Ramesh, D. Kowshik, E. Manisha, K. Manisha, G. Kiranmayi    Bomma Institute of Pharmacy, Khammam, Telangana, India A B S T R A C T The chromatographic conditions were successfully developed for the separation of Pantoprazole by using thermosil C18 4.5×150 mm 5.0 µm, flow rate was 0.8ml/min, mobile phase ratio was 65:35% v/v methanol:water, detection ... Read More »

Phytochemical Studies of Grewia umbelliferrea in Different Solvent Fractions

Vijay Thirugnanasambandan* Department of Biochemistry, Faculty of medicine, Ibn Sina National college for Medical studies, Gulail, Jeddah 21418, KSA A B S T R A C T The present study attempt to evaluate the phytochemical studies of Grewia umbelliferrea stem crude drug. The phytochemical screening was carried out and the berberine alkaloid was quantified in different fractions of extract by HPLC. The ... Read More »

Analytical Method Development and Validation for the Simultaneous Estimation of Candesartan cilexitil and Hydrochlorothiazide by RP-HPLC Method in Bulk and Pharmaceutical Dosage Form

Idavalapati Chandana*, M. Gobinath, V. Haribaskar, Ramesh Dhani Department of Pharmaceutical Analysis, Ratnam Institute of Pharmacy, Pidathapolur, Nellore, Andhra Pradesh, India A B S T R A C T The chromatographic conditions were successfully developed for the separation of Candesartan cilexitil and Hydrochlorothiazide  by using zodiac sil C18 Column (150mm x 4.6mm)5µm, flow rate was 1ml/min, mobile phase ratio was ... Read More »

Analytical Method Development and Validation for the Estimation of Efavirenz by RP-HPLC Method in Bulk and Pharmaceutical Dosage Form

Gobala Krishnan P*1, Munisekhar V, Ranjit Singh B. Rathore Department of Pharmaceutical Chemistry, Vagdevi college of Pharmacy & Research centre, Nellore, Andhra Pradesh, India. A B S T R A C T The chromatographic conditions were successfully developed for the separation of Efavirenz  by using KromosilColumn (100-5 C18.60×4.6mm), flow rate was 1ml/min, mobile phase ratio was Acetonitril: Phosphate buffer PH ... Read More »

Analytical Method Development and Validation for the Estimation of Betahistine HCL by RP-HPLC Method in Bulk and Pharmaceutical Dosage Form

B. Karuna Devi1, G. Sravan Reddy2, SK. Madeesh*3, V. Raja Kumar4, Ramanjineyulu Gunji5 1Malla Reddy College of Pharmacy, Maisammaguda, Dhulapally, Kompally, Medchal, Hyderabad-500014 2CEO, KP Technologies, Kothapet, Hyderabad- 500035 3Manager, Analytical Research and Development, KP Labs, Kothapet, Hyderabad- 500035 4St. John S College of Pharmacy, Yellapur, Hasanparthy, Warangal 3Research Associate, Analytical Research and Development, KP Labs, Kothapet, Hyderabad- 500035 A ... Read More »

Analytical Method Development and Validation for the Simultaneous Estimation of Acyclovir and Hydrocortisone by RP-HPLC Method in Bulk and Tablet Dosage Form

CH. Shankar*, B. Rajesh Department of Pharmaceutical Analysis, Vikas College of Pharmaceutical Sciences, Rayanigudem, Suryapet, Nalgonda, Telangana, India-508376 A B S T R A C T The chromatographic conditions were successfully developed for the separation of Acyclovir and Hydrocortisone by using Agilent C18  Column (250mm x 4.6mm)5µm, flow rate was 1ml/min, mobile phase ratio was  Phosphate buffer PH 2.5:Methanol (65:35 ... Read More »

Analytical Method Development and Validation for the Simultaneous Estimation of Brinzolamide and Timolol Maleate by RP-HPLC Method in Bulk and Tablet Dosage Form

CH. Shankar*, Punna Venkateshwarlu Department of Pharmaceutical Analysis, Vikas College of Pharmaceutical Sciences, Rayanigudem, Suryapet, Nalgonda, Telangana, India-508376 A B S T R A C T The chromatographic conditions were successfully developed for the separation of Brinzolamide and Timolol Maleate by using C18  Column (150mm x 4.6mm)5 µm, flow rate was 1 ml/min, mobile phase ratio was Methanol: Phosphate buffer ... Read More »

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