SYNTHESIS AND CHARACTERIZATION OF 4-[2′-(5′-NITRO) IMIDAZOLYL] BENZOYL (N-ME) VALINE

About author :
Ramesh Dhani
Dept of  Pharmaceutical Chemistry
JNTUA-OTRI, Anantapur, India
E-mail : [email protected]

ABSTRACT:
Many of the heterocyclic found to exhibit antifungal, antibacterial, cytotoxic, antineoplastic, insecticidal, anti-inflammatory, tyrosinase inhibitory and melanin production inhibitory activities. Imidazole has been drawn as promising structural units in the field of medicinal chemistry. Introduction of D-amino acids and N-methylation of amino acids like tyrosine, valine, alanine etc enhanced antimicrobial activity. Hence an attempt is made towards the sythesis of 5-nitroimidazolyl-benzoic acid derivative of N-methyl amino acids and peptide using solution phase technique of peptide synthesis. The new compounds are characterized by using IR, H¹NMR and mass spectroscopy.
Key words: Tyrosine, Valine, Alanine, Benzoic Acid, Imidazole.
Introduction :
A great number of drugs are heterocyclic compounds, mostly are of synthetic origin, few have obtained from natural resources which include alkaloids, xanthines, cardiac glycosides, vitamins and several antibiotics. Heterocyclic derivatives having two nitrogen atoms oriented in, 1-3 position are endowed with wide spectrum of biological activities. Number of organo-sulphur and nitrogen containing compounds are present in living and non living system. Nitroimidazole derivatives were developed not only because of their novelty to structure but also because of novelty to action. N-methylated amino acids are commonly found in naturally occurring peptide antibiotics.  The methylation of N-atom and the hydrogen bonding pattern of peptide containing these amino acids are different from that of unmethylated peptide.  The N-methylated peptide antibiotics are found to possess enhanced activity ascompared to the unmethylated forms.Therefore, an attempt is made to synthesize a new bioactive series of 5-nitroimidazole derivatives of amino acids and peptides. Biological activity studies performed on these synthetic compounds proved to give good results.
Experimental :
1.Synthesis of N-methyl amino acids methyl ester:
Amino acids were converted into the corresponding methyl ester hydrochloride using thionyl chloride and methanol. The amino end was then protected by introducing Boc-group using ditertiary butylpyrocarbonate and triethylamine to get Boc-L-amino methyl ester.  N-methylation of this compound was done by treating with methyl iodide and sodium hydride (Benoition method) to get Boc-(N-Me) amino methyl ester.
2.Preparation of4-[2-(5-nitro)imidzolyl] benzoic acid :
A mixture of p-amino benzoic acid (34.25 gms, 250 mmol), dilute hydrochloric acid (15%, 120ml) and water (150ml) was heated to get a clear solution. The solution was cooled to RT and diazotized by the addition of sodium nitrite solution (30%, 48ml). The diazonium salt solution was filtered and to the filtrate, dilutes HCl (100ml) and nitroimidazole (250 mmol) and aqueous cupric chloride (5gms in 20ml of water) were added with stirring. Stirring was continued for 6 hrs and kept overnight in the refrigerator. The separated solid was collected by filtration and washed with water. The crude compound was crystallized from acetone to obtain pure of 4 [2-(5-nitro)imidazolyl] benzoic acid.
3.Preparation of 4-[2‘-(5‘-nitro) imidazolyl]benzoyl(N-Me)-aminoacid
To the (N-Me) amino acid methylester(7.0 mmol.) THF (20ml), added 4-[2′-(5′-nitro) imidazolyl] benzoic acid acid (1.631gms, 7.0 mmol.), DIPC, Et3N (2.8ml) and stirred at room temp.for 24hr. The reaction mixture was filtered and the filtrate was concentrated under reduced pressure, residue was dissolved in CHCl3, washed with 10% NaHCO3 (10ml) and 5% HCl (10ml), dried over anhydrous Na2SO4 and evaporated under vacuum to get the title compounds. The crude product was recrystallized from CHCl3 and n–hexane.
Results :
The synthesized new amino acid derivatives further studied for characterization of IR, NMR and Mass spectra’s. To study the structure-activity relationship and to optimize the structure.
1HNMR (300 MHz, CDCl3) δ in ppm :
δ 7.7(2H, d, Aromatic-H ),δ 7.6 (2H, d, Aromatic-H),δ 7.15 (1H, s, Aromatic-H),δ 6.2 (1H, br.s, NH2) δ 4.6 (1H, m, α-H) ,δ3.8 (3H, s,-OCH3) δ2.9 (3H, S,-NCH3),δ1.2 (1H, m, β-H),δ0.85 (6H, d, CH3 group of Valine).
IR (CHCl3) in cm-1:
Peak at 3292.1 corresponds to NH and Aromatic C-H stretching, peak at 2931.1 corresponds to Aliphatic C-H stretching; peak at 2855.4 corresponds to Aliphatic C-H stretching, peak at 1701.5 corresponds to C=O (carbonyl) stretching, peak at 1628.9 corresponds to C=O (amide) stretching,Peak at 1543.6 corresponds to N-H bending, peak at 1487.3 corresponds to C-H bending.
Mass in m/z :
M/z: 361 corresponds to molecular ion peak
Conclusion :
The new 4-[2′-(5′-nitro) imidazolyl] benzoyl (N-Me) amino acid derivatives was synthesized and characterized by IR, NMR and Mass spectral data. By this studies find the structure-activity relationship and to optimize the structure. The synthesized amino acid derivative i.e., 4-[2′-(5′-Nitro) imidazolyl] benzoyl N-Me) valine was confirmed by physicochemical & spectral analysis.