Thursday , 13 May 2021

Stastical Comparision, Method Development and Validation of High Performance Liquid Chromatography for estimation of Cifixime Trihydrate and Potassium Clavunate in bulk and combine tablet dosage form

1A. B. Patel*, 2Dr M. M. Patel, 3Dr B. N Suhagiya, 4Dr B. V. Patel, 5Dr. A. B. Patel 
1Assistant Professor, Department of pharmaceutical chemistry, Kalol institute of Pharmacy, Kalol, India
2Director and   Principal of Shankersinh Vaghela Bapu Institute of Pharmacy, India
3Dean at Faculty of Pharmacy, Dharmsinh Desai University, Nadiad
4Professor, Department of Microbiology, Guajrat University, India
5 College of Dental Sciences and Research Centre, Ahmedabad, India

High performance liquid chromatography for estimation of Cifixime Trihydrate and Potassium Clavunate in their combine dosage form was developed and validated. The method was performed on Younglin Instrument with Autochro-3000 Operation software using Varian C-18 (250 Χ 4.6 mm i.d, 5 μm particle size column and Methanol: water: Potassium dihydrogen phosphaste Buffer (pH 3 ) (30:05:65, %v/v/v) as mobile phase at ambient temperature. Detection was carried out at 288 nm. Concentration range 16-80 µg/ml for Cefpodoxime Proxetil and 10-50 µg/ml for Potassium Clavunate. The percentage recovery of Cefixime Trihydrate and Potassium Clavunate Was found to be 99.57-101.16 and 98.91-103.48  respectively.. Correlation coefficient for Cefixime Trihydrate and Potassium Clavunate Sodium was found 0.998 and 0.998 respectively. The Rt values for Cefixime Trihydrate and Potassium Clavunate were found to be 5.1 min ±0.02 and 2.5 min ±0.03 respectively. The method can successfully applicable to routine analysis.  And under stastical analysis ,Paired t-test is applided for comparison between developed method and reported method where, we reject the null hypothesis, because value of t is less than 0.05 so we can conclude that there is significance difference between the developed method and reported method  for Cifixime Trihydrate and and Potassium Clavunate.
Keywords: Cifixime Trihydrate, Potassium Clavunate, HPLC, Mobile phase, Column

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