Jyoti Vilas Jadhav*, Kishorkumar Burade
Govt. College of Pharmacy, Vidyanagar Karad, Satara, Maharashtra- 415124, India
Two validated UV spectrophotometric methods for the simultaneous estimation of Valsartan and Hydrochlorothiazide in pure powder and in two component tablet dosage form have been developed, utilising simultaneous equation and absorbance ratio method. The method is based on the measurement of absorbance of Valsartan and Hydrochlorothiazide at their respective wavelengths of 250.2 nm and 271.6 nm and at the isoabsorptive wavelength of 258.8 nm in a mixed hydrotropic blend of 25% w/v urea and 25% w/v sodium citrate. Linearity was observed in the concentration range of 4-28 µg/ml and 4-32 µg/ml for Valsartan and 2-14 and 4-32 µg/ml for Hydrochlorothiazide by method A and B respectively. The proposed methods have been applied successfully to the analysis of cited drugs in pharmaceutical formulations. Recovery study was performed to confirm the accuracy of the methods. The methods were validated as per ICH guidelines.
Keywords: Valsartan, Hydrochlorothiazide, mixed hydrotropic blend, validation, simultaneous equation method, Q absorbance method