Tuesday , 7 July 2020

RP-HPLC Method Development and Validation For Simultaneous Estimation of Aspirin and Clopidogrel In Bulk and Pharmaceutical Dosage Forms

B. Archana1, Dr. Gampa Vijay Kumar2*
1KGR Institute of Technology and Management, Rampally, Kesara, Rangareddy, Telangana, India.
2Professor and Head, Dept. of Pharmacy, KGR Institute of Technology and Management, Rampally, Kesara, Rangareddy, Telangana, India.

A B S T R A C T
The proposed HPLC method was found to be simple, specific, precise, accurate, rapid and economical for simultaneous estimation of AspirinAcetaminophene and Clopidogrel in tablet dosage form. The developed method was validated in terms of accuracy, precision, linearity, robustness and ruggedness, and results will be validated statistically according to ICH guidelines. The Sample recoveries in all formulations were in good agreement with their respective label claims. From literature review and solubility analysis initial chromatographic conditions Methanol: Ammonium acetate PH3 (70:30)were set adjust Ph up to 3 with ortho phosphoric acid Agilent C18 (4.6 x 150mm, 5mm)Column, Flow rate 1.0 ml/min and temperature was ambient, eluent was scanned with PDA detector in system and it showed maximum absorbance at 254 nm. As the methanol content was increased Aspirin and Clopidogrel got eluted with good peak symmetric properties. The retention times for Aspirin and Clopidogrel was found to be 2.972 min and 3.548 min respectively. System suitability parameters were studied by injecting the standard five times and results were well under the acceptance criteria. Linearity study was carried out between50% to150 % levels, R2 value was found to be as 0.999.
Keywords: Agilent C18, Aspirin and Clopidogrel, HPLC

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