Method Development and Validation of Valacyclovir in Pharmaceutical dosage form by RP-HPLC Method

Pavan Kumar V*¹, Dr. C. Sreedhar¹, Pavan Kumar Sai.B², Haritha.Y³, Manisha.N⁴, Venkateswarlu G^4
1Department of Pharmaceutical Analysis, Karnataka College of Pharmacy, Bengaluru, Karnataka
²Department of Pharmaceutical Analysis & Quality Control, Alliance Institute of Advanced Pharmaceutical and Health Sciences, Hyderabad, Telangana.
³Department of Pharmaceutics, Gokula Krishna College of Pharmacy, Sullurpet, Nellore (DT), Andhra Pradesh.
⁴Aurobindo Pharma Limited, Hyderabad, Telangana, India

A B S T R A C T
A Simple, accurate, precise and reproducible reverse phase high performance liquid chromatographic (RP-HPLC) method was developed for the analysis of Valacyclovir in pharmaceutical dosage form. The method development was carried out by using Hypersil-C₁₈, 250x 4.6mm, 5μ column as stationary phase with a mobile phase of Methanol:Orthophosphoric acid (60:40) v/v at a flow rate of 1 ml/min, injection volume was 20μl,column temperature was ambient, retention time was 3.007min and UV detector wavelength was 253nm. Linearity of this method was in the range of 20-70μl,the correlation coefficient was found to be 0.999.The developed method was validated for Linearity, Accuracy, Precision, Sensitivity and Robustness. All the validated parameters were within the limit. The proposed method was found to be simple, economic, fast, accurate, precise and hence can be used for the routine analysis of Valacyclovir in pharmaceutical dosage forms.
Keywords: Valacyclovir, RP-HPLC, Hypersil-C₁₈ column, Method development and validation.