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Valluru Vijayamma*, M. Gobinath, V. Hari Baskar, Ramesh Dhani, V. Pavan Kumar, SK. Kowsar
Department of Pharmaceutical Analysis, Ratnam Institute of Pharmacy, Pidthapolur, Muthkur, Nellore-524346, A.P, India
A B S T R A C T
A simple, Accurate, precise method was developed for the simultaneous estimation of the Olmesartan Medoxomil and Hydrochlorothiazide in Tablet dosage form. Chromatogram was run through Inertsil -ODS C18, 250 x 4.6 mm, 5m. Mobile phase containing Methanol and Buffer in the ratio of 80:20 was pumped through column at a flow rate of 1ml/min. Optimized wavelength for Olmesartan Medoxomil and Hydrochlorothiazide was 260nm. Retention time of Olmesartan Medoxomil and Hydrochlorothiazide were found to be 3.270min and 4.566 min. %RSD of the Olmesartan Medoxomil and Hydrochlorothiazide were and found to be 1.56 and 1.01 respectively. %assay was obtained as 99.91% and 99.95% for Olmesartan Medoxomil and Hydrochlorothiazide respectively. LOD, LOQ values are obtained from regression equations of Olmesartan Medoxomil and Hydrochlorothiazide were 0.22ppm, 0.25ppm and 0.69ppm, 0.75ppm respectively. Regression equation of Olmesartan Medoxomil is y = 30712x – 31891, and y = 16499x + 8683 of Hydrochlorothiazide.
Keywords: Olmesartan Medoxomil, Hydrochlorothiazide, RP-HPL
