Sunday , 16 May 2021

Experimental Study of M. oleifera Leaf Extract in Aqueous and Powder Formulations on Immune Response of Chicks by Nitroblue Tetrazolium Reduction Test

Swechha Akhouri1, Arun Prasad1, Subha Ganguly2*
1Department of Veterinary Microbiology, College of Veterinary Science & Animal Husbandry, Birsa Agricultural University, Kanke, Ranchi – 834 006, Jharkhand, India
2AICRP-PHT (ICAR), Department of Fish Processing Technology, Faculty of Fishery Sciences, West Bengal University of Animal and Fishery Sciences, Panchasayar, Chakgaria, Kolkata – 700 094, WB, India

In the present study, the aqueous extract and dried powder of Moringa oleifera leaf was investigated for its immunostimulatory effect in broiler chick. It was evaluated on the basis of percentage of formazan positive cell in blood samples and skin sample collected at the end of reaction. Fifty (50 no.) day-old chicks were kept into 5 groups each contain 10 chicks.  First two treatment groups (T1 and T2) were fed with aqueous extract and dried powder of M. oleifera respectively each @ 250 mg/kg b.wt. For comparison, a levamisole fed group T3 was included, to compare the immunomodulatory effect of the preparations of M. oleifera with that of a known positive immunomodulator, which is fed @ 10 mg/kg b.wt. The other two groups T4 and T5 included in the experimental design served as the vaccinated and unvaccinated control groups respectively. The effect of treatment was recorded from day 14th   day. From day 14 up to day 42, M. oleifera treated groups showed stimulatory effect on non-specific immune response which is judged by relative percentage of formazan positive cells in blood sample. No mortality was recorded in the treated groups. M. oleifera showed stimulatory effect on non-specific immune response in chicks.
Keywords: Formazan positive cells, Immunostimulatory effect, Moringa oleifera

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