Bariya Aditi Hemrajbhai1*, Patel Madhabhai Manohardas2, Anand Indermeet Singh3
1Assistant Professor, Department of Pharmacology, Kalol Institute of Pharmacy, Kalol-382721, Gujarat, India.
2Principal & Director, Shankersinh Vaghela Bapu Institute of Pharmacy, Vasan, Gandhinagar, Gujarat, India.
3Professor & Head, Department of Pharmacology, Shree Sarvajanik Pharmacy College, Mehsana, Gujarat, India.
Terminalia arjuna wight & Arn. is a deciduous and evergreen tree, belongs to Combretaceae family. In the present study, Terminalia arjuna was tested for 2, 4 – dinitro benzene sulfonic acid (DNBS) induced colitis, and its antioxidant & anti inflammatory activities were evaluated to clarify its possible mode of action. Male albino wistar rats were randomly divided into six groups: Normal control (Group I), Model control, colitis induced by DNBS without any therapy (Group II), Vehicle control (Group III), Standard control, treatment with Dexamethasone (Group IV), Treatment with Ethanolic extract of T.arjuna (Group V) Treatment with Aqueous extract of T.arjuna (Group VI). Treatment was given for 20 days. Rats were sacrificed on the 20th day after the procedure. Malondialdehyde (MDA), Nitric oxide (NO), Reduced Glutathione (GSH), Superoxide dismutase (SOD), activity were measured in the isolated colon tissue. MDA & NO levels in colon tissue homogenate were decreased & GSH, SOD levels were increased in Group IV, Group V & Group VI as compare to those of Group II. There was also increase in food intake, water intake, % body weight & decreased colon weight in Group IV, Group V & Group VI as compared to Group II. There was also improvement in inflammatory indices of colon mucosal damage index (CMDI) & Disease activity index (DAI) & histopathology of Group IV, Group V & Group VI as compared to those of group II. The results of our study suggest that T.arjuna therapy has beneficial effects on the course of experimental colitis.
Keywords: Terminalia arjuna, Inflammatory bowel disease (IBD), Antioxidant, Anti inflammatory