Thursday , 19 May 2022

Evaluation of in-vitro Antitumor Property of Water Weeds (Eichhorniacrassipes and Pistiastratiotes) by MTT Assay

Poonam Sharma1, N. Ganesh*1, Vinoy. K. Shrivastava2
1Department of Research, Jawaharlal Nehru Cancer Hospital & Research Centre, Idgah Hills, Bhopal, Madhya Pradesh
2Department of Biosciences, University Teaching Department, Barkatullah University, Bhopal

The water weeds Eichhorniacrassipes and Pistiastratiotes were selected to investigate the antitumor property in-vitro by MTT Assay. The IC50 (IC-Inhibitory Concentration) was calculated for all the fractions of the selected aquatic plants isolated through column chromatography. The fractions were subjected for HPLC analysis. At higher concentration of drug (500mg) less viable cells were found. Pistia have shown better cytotoxicity than Eichhornia when subjected to B16F10 cells, the % of viable cells scored at 500mg of aqueous fractions was 26 and 35 respectively. The IC50 of HPLC eluted fractions of Eichhornia&Pistia was found between 200 and 500mg/ml but the crude extracts of these water weeds did not respond similarly. The crude and alcoholic fractions of Eichhorniacrassipes and aqueous and methanolic fractions of Pistiastratiotes proved as potential tumor inhibitor.
Keywords: MTT, cytotoxicity, antitumor, in-vitro.

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